Coding

Part:BBa_K3452000:Design

Designed by: Hao Zhou   Group: iGEM20_CSU_CHINA   (2020-10-26)


hMT-1A


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 946
    Illegal SpeI site found at 1135
    Illegal PstI site found at 1155
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 946
    Illegal SpeI site found at 1135
    Illegal PstI site found at 1155
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 862
    Illegal BglII site found at 1051
    Illegal BamHI site found at 673
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 946
    Illegal SpeI site found at 1135
    Illegal PstI site found at 1155
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 946
    Illegal SpeI site found at 1135
    Illegal PstI site found at 1155
    Illegal AgeI site found at 724
    Illegal AgeI site found at 892
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization is needed so that it can be expressed more successfully in the Synechocystissp.


Source

Homo sapiens

References